Part:BBa_K3286206:Design

RpfCch sfGFP fusion

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 227
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 757
  Illegal NgoMIV site found at 848
  Illegal NgoMIV site found at 1675
  Illegal NgoMIV site found at 1770
  Illegal AgeI site found at 2326
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

To fuse these parts, the stopcodon of RpfCch and the startcodon of the sfGFP were removed and than fused with Golden Gate.

Source

See the BBa_K3286200, BBa_K3286203 parts for further information on part source.